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Collagen deposition patterns were observed from picrosirius red-stained cross sections, viewed under polarized light. There was increased new collagen in diabetes compared with control (increased green staining), and metformin treatment prevented this effect. Representative images (A) and combined analyses are shown of mature (B) and new collagen (C) for all animals. *P < 0.05 vs. control or euglycemic; n = 5/group.
