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Expression of type 1 collagen (A), collagenase (B), and MMP-13 (C) were determined by slot-blotting and immunoblotting, respectively. Densitometric analysis showed increased expression of type 1 collagen and MMP-13 in diabetic animals. B: increased fluorescence that is directly proportional to the proteolytic cleavage of FITC-collagen by MMP-13 was measured at 24 h, and it was decreased in diabetic animals. *P < 0.05 vs. control, n = 6–8/group.
