13C NMR spectrum of carbon-2 resonance of MAG derived from plasma glucose. Fluxes of MAG were derived from plasma glucose from an overnight fasted obese and a lean cat, respectively, after infusion with [3,4-13C2]glucose and oral administration of 2H2O and [U-13C2]propionate. The spectrum shows a doublet from coupling of carbon-1 with carbon-2 (D12), a doublet from coupling of carbon-2 with carbon-3 (D23), a doublet of doublets (quartet; Q) arising from coupling of carbon-2 with both carbon-1 and -3, and a singlet resonance (S).