Fig. 1.

Schematic line drawing illustrates the design of different Halász's knife isolations. Microknife isolations started at −1.8 mm from bregma in the case of the bilateral anterolateral cut (ALC) and unilateral-anterolateral cut (UALC) isolation groups. Mid-anterior cut (ACmid) started at −2.3 mm posterior from bregma and the posterior-anterior cut (ACpost) started at −2.8 mm posterior from bregma. Pituitary stalk lesion (PSL) cuts started at −4.2 mm posterior to bregma. When the tip of the knife reached the base of the skull the knife was rotated 90° to both sides in the case of the ALC, ACmid, ACpost, and PSL isolation groups. In the case of the ALC group, cuts were further elongated backward in the lateral direction. The UALC group of rats were prepared using the same procedure as the ALC group, but this group was cut only unilaterally (either left or right side). Other isolation groups were not elongated backward in the lateral direction. 3V, third ventricle; Apit, anterior pituitary; ARC, arcuate nucleus of the hypothalamus; DMH, dorsomedial nucleus of the hypothalamus; LH, lateral hypothalamus; ox, optic chiasm; PeV, periventricular nucleus of the hypothalamus; Ppit, posterior pituitary; Rch, retrochiasmatic area; SCN, suprachiasmatic nucleus; SO, supraoptic nucleus; VMH, ventromedial hypothalamic nucleus.