Table 1.
Primer sequences (5′ to 3′) used for qRT-PCR
| Gene | General Description | Forward | Reverse | Accession No. |
|---|---|---|---|---|
| mRNAs Associated with mTOR | ||||
| REDD1 | negative regulator of mTOR | CTGGAGAGCTCGGACTGC | TCCAGGTAAGCCGTGTCTTC | NM_019058 |
| REDD2 | negative regulator of mTOR | CCCAGAGAGCCTGCTAAGTG | TTGCTTTGATTTGGACAGACA | NM_145244 |
| TSC1 | negative regulator of mTOR | TAGGCTGGAGGGACTGTGAG | CCTGGTGTCTTTCATGGTCA | NM_000368 |
| TSC2 | negative regulator of mTOR | CCGCAGCATCAGTGTGTC | CACTGGTGAGGGACGTCTG | NM_000548 |
| Rheb | positive regulator of mTOR | TTTTTGGAATCTTCTGCTAAAGAAA | AAGACTTGCCTTGTGAAGCTG | NM_005614 |
| TCTP | positive regulator of mTOR | GTCGTCGTCTCCCTTCAG | AGCGAGTCATCAATGTTACC | NM_003295 |
| *cMyc | downstream target of mTOR; positive regulator of ribosomal biogenesis | CGTCTCCACACATCAGCACAA | TGTTGGCAGCAGGATAGTCCTT | NM_002467 |
| PRAS40 | negative regulator of mTOR | GAACGGATAAGAGAAGAGG | CGGTGACTCAGAAATTCG | NM_032375 |
| hVps34 | nutrient regulator of mTOR | GAAGCAGATGGATCAGAACC | CCAGCCAATCTACTTTCACC | NM_002647 |
| MAP4K3 | nutrient regulator of mTOR | AGCCCAGCAAAGCCATCC | GCCATCTCGTTCACCATTTAAC | NM_003618 |
| *mTOR | positive regulator of protein synthesis | TCGTGCCTGTCTGATTCTC | GATTCATGCCCTTCTCTTTGG | NM_004958 |
| *S6K1 | downstream target of mTOR; positive regulator of protein synthesis | ACTCAATTTGCCTCCCTACC | AGAATGGATGAGCTTGAACTTC | NM_003161 |
| mRNAs Associated with Satellite Cell Function | ||||
| mib2 | E3-ligase for notch signaling | CTACGAGACCGCTCACTC | CCTTCGCTCCCTGGAAAATG | NM_080875 |
| *myostatin | negative regulator of satellite cell proliferation | CCGTCGAGACTCCTACAAC | ACACTGTCTTCACATCAATGC | AF019627 |
| *IGF-1R | promotes satellite cell activation and proliferation | ATGCGGTGTCCAATAACTAC | TTGTTGATGGTGGTCTTCTC | NM_000875 |
| *myoD | promotes satellite cell proliferation | GGTCCCTCGCGCCCAAAAGATT | CAGTTCTCCCGCCTCTCCTACCTCAA | NM_002478 |
| *myogenin | promotes satellite cell differentiation | TGGAGCTGTATGAGACATCC | CTCGTAGCCTGGTGGTTC | NM_002479 |
| Housekeeping Genes | ||||
| *GAPDH | housekeeping gene | AGGTGAAGGTCGGAGTCAAC | GCTCCTGGAAGATGGTGATG | J02642 |
| β2M | housekeeping gene | CCAAAGATTCAGGTTTACTCAC | TCAACTTCAATGTCGGATGG | NM_004048 |