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Lipolytic activity demonstrated by cleavage of polyoxyethylene sorbitan monolaurate (Tween 20). A) CULPs with activity for pNPP were tested at varying pH. B) Culp6 activity for Tween 20 performed from pH 5.5–8.5. Bars represent means ± se of triplicate experiments. C) Comparison of Culp6 activity (U/μg protein) to that of a purified lipase of Candida rugosa (Cr). Data points are means ± se of triplicate experiments.
