TABLE 2.
Enzymatic reaction products detected in assays for cutinase activity of M. tuberculosis CULPs
| Cutin hydrolysis product | Reaction time (min) | Hydrolysis area (%)
|
|||||||
|---|---|---|---|---|---|---|---|---|---|
| T. fusca cutinase | Culp1 | Culp2 | Culp3 | Culp4 | Culp5 | Culp6 | Culp7 | ||
| Hexadecanoic acid | 6.926 | 30.94 | 24.25 | 37.92 | 25.11 | 40.26 | 32.44 | 27.09 | 23.18 |
| 9-Octadecenoic acid | 9.1 | 0.912 | 1.55 | 5.1 | 0.512 | 0.571 | 1.757 | 0.349 | 0.492 |
| 9,12-Octadecadienoic acid | 9.188 | 1.696 | 1.825 | 0.345 | 0.316 | 2.166 | 2.143 | 0.913 | 0.554 |
| Octadecanoic acid | 9.525 | 36 | 32.16 | 39.06 | 31.08 | 44.36 | 41.73 | 32.59 | 27.68 |
| 16-Hydroxyhexadecanoic acid | 11.547 | 0.843 | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. |
| 18-Hydroxyoctadeca-9-enoic acid | 13.017 | 1.97 | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. |
| 18-Hydroxyoctadeca-9,12-dienoic acid | 13.558 | 7.18 | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. |
| 10,16-Dihydroxyhexadecanoic acid | 13.99 | 5.8 | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. | N.D. |
N.D, not determined.