Figure 1.

Detection of mouse RAGE splice variants. A) Total PCR product for all different clones detected from the mouse lung, kidney, brain, and heart cDNA for RAGE. B) Restriction map of full-length RAGE cDNA with BamHI and PstI. Restriction sites are shown by arrows in the respective exon where they occur. Resulting DNA fragments are shown in base pairs; boxes represent exons. Primers used are indicated by half arrows. C) Restriction digest of the mouse RAGE cDNA PCR products with BamHI and PstI. The corresponding splice variant classification is shown above the digestion. DNA fragments were sized against a 1-kb DNA ladder as indicated on each gel.