TABLE 2.

Lack of effect of patient MAW purified Igs on S1P binding to S1P₁

	Normal Igs (μg/ml)			Patient MAW Igs
	40	200	1000	40	200	1000
Mean % of control	107a	107	105	98	105	105

Each replicate was incubated with 200 fmol of ³²P-S1P (mean cpm=44,595) without and with a 1000-fold molar excess of nonradioactive S1P. In the absence of Igs, mean specific binding of S1P by S1P₁-HEK293 cell transfectants was 16.9 fmol over a background of 1.0 fmol for HEK293 cells transfected with vector alone. 

^aMean value for one of three identical studies showing no significant effect of Igs on binding.