Figure 5.

Lipidomic analysis of adipose tissue samples using LC/MS/MS. A) Levels of lipid mediators generated in adipose tissue of untreated ob/ob mice. Samples were extracted in C18-ODS solid-phase columns and analyzed by LC-MS/MS-based lipidomics (see Materials and Methods for details). B) Percentage change of tissue levels of ω-3-PUFA-derived docosanoids and ω-6-PUFA-derived eicosanoids in mice receiving ω-3-PUFA-enriched diet (n=8) compared to those receiving CT diet (n=4). Results are expressed as means ± se. *P < 0.05, **P < 0.005, ***P < 0.0005 vs. CT group.