Figure 6.

Effects of resolvins and protectins on liver and adipose tissue from ob/ob mice. A) Representative photomicrographs of liver sections stained with Oil Red O (top) or incubated with an F4/80 specific antibody (bottom) in ob/ob mice receiving intraperitoneal saline as a control (n=5) or resolvin E1 (RvE1) (n=5) for 4 d. B) Adiponectin, GLUT-4, IRS-1, and PPARγ mRNA expression in adipose tissue samples obtained from ob/ob mice treated with RvE1 mice was determined by real-time RT-PCR. C) Adipose tissue explants from ob/ob mice were incubated for 12 h in the presence of vehicle (V; 0.5% EtOH), protectin D1 (PD1; 100 and 250 nM), or rosiglitazone (10 μM). Four different experiments with duplicates were performed. At the end of the incubation period, RNA was extracted, and adiponectin expression was determined by real-time RT-PCR. Results are expressed as means ± se; P values vs. CT group (A, B) or V group (C).