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. Author manuscript; available in PMC: 2009 Jun 18.
Published in final edited form as: Nat Struct Mol Biol. 2008 Mar 30;15(4):354–363. doi: 10.1038/nsmb.1409

Figure 1.

Figure 1

Both miRNA and miRNA* species can be detected in total RNA. RNA was analyzed from D. melanogaster cells at different stages: E, 0–24 h embryos; LP, 3rd instar larvae and mixed pupae; A, adult males and females; S, S2 cells. Each blot was sequentially hybridized, stripped and rehybridized to detect a miRNA* species, its partner miRNA species, then 2S rRNA (the exception is miR-10*, whose companion 2S blot is shown in Supplementary Fig. 1). Arrows indicate the mature 21–24 species and brackets indicate the pre-miRNA hairpin.