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. Author manuscript; available in PMC: 2009 Jun 18.
Published in final edited form as: Nat Struct Mol Biol. 2008 Mar 30;15(4):354–363. doi: 10.1038/nsmb.1409

Figure 2.

Figure 2

Highly conserved miRNA* species accumulate to higher relative levels at steady state. (a) Evolution of the mir-309->mir-6 cluster. Both miRNA (green) and miRNA* (yellow) sequences of mir-4 and mir-5 are perfectly conserved across 12 Drosophilids. The other six miRNA* species in this cluster all accumulated divergence (red nucleotides); mir-6-1 is shown. (b) Temporal dynamics of miRNA:miRNA* ratios. Six genes show read ratios that rise progressively with embryo age (time after egg laying (AEL)). Only the genes with highly conserved miRNA* (mir-4 and mir-5) maintain a low miRNA:miRNA* ratio. (c) The miRNA:miRNA* ratios of all 26 miRNA loci that generate at least 50 clones in each of four embryonic time points. Loci whose miRNA* species is perfectly conserved in 11 or 12 Drosophilids are in blue; all other loci are in gray. The most highly conserved miRNA* species tend to be present at a more comparable level to their miRNA partners and are concentrated at the bottoms of these graphs.