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. Author manuscript; available in PMC: 2009 Jun 18.
Published in final edited form as: Dev Cell. 2007 Feb;12(2):221–234. doi: 10.1016/j.devcel.2006.12.003

Figure 2. Comparison of whole mount Bim−/− and control mammary glands during puberty.

Figure 2

(A–B) Whole mount staining of mammary glands from five week-old Bim+/+ (A) and Bim−/− (B) mice at low (left panel) and high (right panel) magnification. The luminal space is filled (white arrow) in the Bim −/− TEB (B, right panel). (C) Morphometric study of Bim−/− TEB at five weeks. Left panel: percentage of TEB without any detectable luminal space in Bim+/+ (4.4 % ± 1.3; n=7) and Bim−/− (86.6 % ± 9.3; n=8) mammary glands at five weeks. Right panel: size distribution (μm2) of terminal ends of the ducts in Bim+/+ (n=4) and Bim−/− (n=4) mammary glands at five weeks. The size distribution of terminal ends in the Bim+/+ and Bim−/− glands is: 66.7%± 7.8 (+/+) and 56.6% ± 6.3 (−/−) for sizes below 20000 μm2, 15.2% ± 1.7 (+/+) and 16.9% ± 2.6 (−/−) between 20000 and 30000 μm2, 8% ± 3.7 (+/+) and 7.6% ± 2.3 (−/−) from 30000 to 40000 μm2; 4.6% ± 1.1 (+/+) and 6.5% ± 1 (+/+) between 40000 and 50000 μm2 (paired t-test with p<0.05); 5.5% ± 2.3 (+/+) and 12.4% ± 3 (−/−) for TEBs above 50000 μm2 (paired t-test with p<0.01) (D–E) Whole mount staining of mammary glands from 6 to 7 week-old wild type (D) and Bim−/− mice (E) at low and high magnification. Note that the luminal space is clearing (black arrow) in E. (F–G) Whole mount staining of eight week-old wild type control (F) and Bim−/− (G) mammary glands at low and high magnification. [Scale bar: 300 (A-B-D-E), 450 (D–E), 550 (F–G), 60 (A-B-D-E-F-G insert), 30 (D–E right inserts) μm]. Abbreviation: TEB (terminal end bud), ln (lymph node).