TABLE 1.
Characteristics of obese mice treated with RA and GW0742 for 3 weeks
| Parameter | Mean ± SD for each mouse treatment groupa
|
||
|---|---|---|---|
| Control mice | RA-fed mice | GW0742-fed mice | |
| General | |||
| wt loss (%) | 5 ± 0.2 | 11 ± 0.4* | 3 ± 0.1 |
| Food intake (treated/control) | 1 | 1.33 ± 0.05** | 1.18 ± 0.04* |
| Rectal temp (°C) | 36.7 ± 0.2 | 37.2 ± 0.2** | 37.1 ± 0.1** |
| Concn in blood | |||
| β-Hydroxybutyrate (mg/dl) | 3.8 ± 0.3 | 3.9 ± 0.3 | 3.9 ± 0.3 |
| Cholesterol (mg/dl) | 200.3 ± 8.4 | 181.8 ± 5.8 | 229 ± 8*** |
| Triglycerides (mg/dl) | 185.8 ± 18.1 | 94 ± 16** | 141.3 ± 14.1‡ |
| Free fatty acids (mM) | 0.50 ± 0.04 | 0.41 ± 0.05 | 0.46 ± 0.04 |
| Glycerol (μM) | 70.0 ± 0.3 | 92.0 ± 0.5* | 90.0 ± 0.4* |
| Insulin (μg/liter) | 4.4 ± 0.5 | 2.0 ± 0.4*** | 3.4 ± 0.3 |
a
Sixteen-week-old mice fed a high-fat/high-sucrose diet were orally fed RA or GW0742 (0.16 mg/day) daily for 3 weeks. Blood was collected after an overnight fast. Plasma was isolated by using Microtainer plasma separator tubes (Becton Dickinson). Insulin was measured by using an ultrasensitive mouse insulin enzyme-linked immunosorbent assay (Mercodia). Other parameters were measured by Veterinary Diagnostic Services (Marshfield Laboratories). Data are means from eight animals. *, P = 0.05; **, P < 0.005; ***, P = 0.02; ‡, P = 0.07 (versus untreated mice).