FIG. 2.

Representative examples of mutant screening data. HEK293 cells were transfected with the indicated Jak2 or Jak2 mutant plasmid. Cells were made quiescent overnight. Cells were either lysed without treatment (0) or incubated in the presence of Epo (12.5 U/ml) for either 15 or 60 min and then lysed. Lysates were immunoprecipitated (IP) with an antibody to the total Jak2. Immunoprecipitates were resolved by SDS-PAGE, transferred to nitrocellulose, and immunoblotted (IB) with the indicated antibodies. The results shown are typical of three independent experiments.