FIG. 5.

Effects of Tyr₃₁₇ and Tyr₆₃₇ on downstream signaling. HEK293 cells were transfected with the indicated plasmids, made quiescent overnight, and incubated in the absence (−) or presence (+) of Epo (12.5 U/ml) for 15 min before lysis. Lysates were resolved by SDS-PAGE, transferred to nitrocellulose, and immunoblotted (IB) with the indicated antibodies. The results shown are typical of multiple independent experiments.