FIG. 5.

Role of Pol η in CFS stability. (A) Localization of FRA7H probe (in green) was analyzed by using FISH in control cells (CTR1) and checked using a centromeric probe (in red) specific to chromosome 7. (B and C) Expression of the CFS FRA7H (translocations, amplifications, deletions) was analyzed by using FISH in Pol η-deficient cells (shη1 and shη2). The quantification of FRA7H expression in cells treated or not treated with aphidicolin (100 nM) for 24 h is presented in panel D (three experiments; >50 metaphases), and standard deviations are indicated by error bars. (E) Colony-forming assay of Pol η-depleted U2OS cells was determined as a function of caffeine concentration. Experiments were done three times, with triplicate samples for each caffeine dose, in order to determine mean numbers of CFUs, and standard deviations are indicated by error bars.