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Pre-miRNA excision from pre-mRNA is kinetically faster than mRNA splicing. In vitro splicing reactions with δ-crystallin-330 pre-mRNA were performed with 10 min or 20 min of incubation. After the reaction, RNA products were analyzed as described in the legend to Fig. 2. The identity of each RNA product is shown schematically at the right. An asterisk shows an aberrant splicing product.
