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. 2009 Apr 6;29(12):3243–3254. doi: 10.1128/MCB.00360-09

FIG. 8.

FIG. 8.

Drosha associates with spliceosomal U snRNPs. Northern blotting analysis was performed to observe the spliceosomal U snRNAs in the immunoprecipitated fractions. Whole-cell extracts from HEK293T cells were subjected to immunoprecipitation with anti-Sm antibody (Y12) (lane 2), anti-Drosha (lane 3), or control antibody (lane 4). Coprecipitated RNAs were detected by Northern blot analysis probing for the five spliceosomal U snRNAs. The input lane contained 5% of the HEK293T whole-cell extract used for immunoprecipitation.