FIG. 2.

E2-induced CARM1 activity at ERα sites associates with activating events. (A) Level of recruitment for the coactivators p300 and SRC1 under vehicle (O) or E2 treatment established by ChIP-qPCR on eight ERα sites associated and eight not associated with CARM1 activation in MCF7 breast cancer cells. (B) Levels of histone modifications, namely, H3K18ac, H3K27ac, and H4K12ac, were determined as in panel A. (C) Impact of E2 treatment on nucleosome density. The changes in occupancy of the core histone H3 were determined by ChIP-qPCR as in panel A. Alterations to the DNA accessibility were determined by using FAIRE (21). The results are derived from a minimum of two independent experiments. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001.