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TCR activation stimulates translocation of C3G from cytosol to membranes of Jurkat T cells. Serum-starved Jurkat T cells were stimulated with or without anti-CD3 antibodies and disrupted by nitrogen cavitation. The cavitated samples were separated into total membranes (P175) and cytosol (S175) and analyzed for C3G and RhoGDI by immunoblotting. Results shown are representative of three independent experiments.
