FIG. 6.

C3G-dependent TCR-stimulated T-cell adhesion to ICAM-1 requires PLD1. (A) Jurkat T cells transfected with GFP-C3G or GFP vector and treated with or without t-butanol or n-butanol were assayed for adhesion to ICAM-1 as described in the legend to Fig. 5, before and after stimulation with anti-CD3 antibodies. (B) Jurkat T cells were transfected with GFP-C3G and the indicated shRNA, and the adhesion of transduced, fluorescent cells to ICAM-1 was determined. (C) Jurkat T cells transfected with GFP-Rap1V12 and treated with or without t-butanol or n-butanol were stimulated or not with anti-CD3 antibodies, and adhesion to ICAM-1 was measured. Results are shown as means ± SEM (n = 3).