FIG. 4.

Forced expression of Drosha does not affect the accumulation of pri-miRNAs by NF90-NF45. (A) RNAs isolated from 293T cells transfected with the indicated expression plasmids were analyzed for expression of pri-miRNAs by qRT-PCR with specific primers for pri-let-7a-1 and pri-miR-21. β-Actin was used as an internal control and for normalization of the data. These results were reproducible in three independent transfection experiments. Data represent the averages of triplicate PCRs with ranges. (B) RNA and WCEs were prepared from 293T cells transfected with the indicated expression plasmids and analyzed by RT-PCR and immunoblot analysis, respectively. Intensities of specific bands in the immunoblotting assay were measured with a densitometer and are presented as relative band intensities.