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. 2009 Apr 27;29(13):3754–3769. doi: 10.1128/MCB.01836-08

FIG. 6.

FIG. 6.

NF90 and NF45 do not interact with endogenous Drosha-DGCR8. (A) WCEs from 293T cells transfected with the indicated expression plasmids were immunoprecipitated with anti-Drosha antibody or normal rabbit IgG. The immunoprecipitates were probed with anti-Drosha, anti-DGCR8, or anti-NF90 antibodies. Arrows indicate the specific target proteins. (B) WCEs from 293T cells transfected with the indicated expression plasmids were subjected to blue native page analysis, followed by immunoblot analysis using anti-Drosha, anti-DGCR8, anti-NF90, or anti-NF45 antibodies. Arrows indicate the complexes containing the specific target proteins.