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. 2009 Apr 27;29(13):3754–3769. doi: 10.1128/MCB.01836-08

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Copyright © 2009, American Society for Microbiology

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FIG. 7. — The NF90-NF45 complex binds to endogenous pri-miRNAs in vivo. The experimental procedure is indicated in the upper part. WCEs from 293T cells transfected with indicated FLAG-tagged proteins were immunoprecipitated with anti-FLAG antibody. RNAs were isolated from the immunoprecipitates and analyzed by RT-PCR with specific primers for pri-let-7a-1, pri-miR-15a∼16-1, pri-miR-21, or pri-miR-30a. The numbers of PCR cycles for detection of pri-let-7a-1 and pri-miR-15a∼16-1, pri-miR-21, and pri-miR-30a were 35 and 45 cycles, respectively.