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Sequence alignment and roles of ATR1 paralogs in boron tolerance. (A) Multiple-sequence alignment of yeast ATR1 paralogs. Conserved residues are highlighted with BOXSHADE 3.21. (B) Comparisons of the boron tolerances of the atr1Δ mutant and paralogs, YMR279c and YOR378w mutants. Overnight cultures were diluted, and 5 μl of each cell suspension was spotted. The cells were grown for 3 days, and the plates were photographed.
