FIG. 6.

Dynamin decreases Pyk2 Y881 phosphorylation and Pyk2's binding to Grb2. (A) 293VnR cells were transiently cotransfected with equal amounts of cDNAs encoding Pyk2 and dyn-GFP, dynK44A-GFP, or empty vector as indicated. Pyk2 was immunoprecipitated, resolved by SDS-PAGE, and blotted with phospho-specific antibodies to Pyk2 tyrosine residues Y402, Y579, Y580, and Y881. TCLs were blotted for Pyk2 or dynamin (GFP) as indicated. (B) 293VnR cells were cotransfected with Pyk2, dyn-GFP or Grb2-myc as indicated. Pyk2 was immunoprecipitated and blotted for Grb2 (myc) or dynamin (GFP). TCLs were blotted to confirm protein expression in these cells. Representative blots from three independent experiments are shown. The positions of molecular mass markers are indicated. Labels: IP, immunoprecipitation; B, Western blot; Dyn, dynamin.