FIG. 9.

Src SH2 and SH3 domains mediate the dynamin-Pyk2 association. 293VnR cells were cotransfected with Pyk2, dyn-GFP, or empty vector in combination with either wild-type c-Src or Src mutants with a disabled SH3 or SH2 domain (SrcW118K and SrcR175L, respectively), as indicated. Immunoprecipitation and Western blots were performed as indicated. Exogenously expressed Src proteins were detected with an anti-avian Src antibody that does not detect endogenous Src. Disabling either the SH3 domain or the SH2 domain significantly reduced the Pyk2-dynamin association (arrows). Molecular mass markers and the position of IgG band are indicated. Labels: IP, immunoprecipitation; B, Western blot; Dyn, dynamin.