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. 2009 Apr 27;29(13):3623–3632. doi: 10.1128/MCB.00229-09

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FIG. 3. — dAbl regulates Kette. (A) Flag-tagged Kette^Myr was ectopically expressed in the absence or presence of HA-tagged dAbl in S2 cells. Immunoprecipitated (IP) Kette^Myr or an aliquot of total lysates was subjected to immunoblotting (IB). (B) S2 cells were treated with dsRNA for dAbl or green fluorescent protein (GFP). dsRNA-treated S2 cells were transfected with Flag-tagged Kette^Myr. Immunoprecipitated Kette^Myr or an aliquot of total lysates was subjected to immunoblotting. (C) Ectopic expression of dAbl resulted in eye roughness. Coexpression of dAbl and Kette^Myr (dAbl; Kette^Myr) significantly enhanced the defect. On the other hand, expression of the catalytically inactive mutant form of dAbl (dAbl^K417N) rescued the rough-eye phenotype induced by Kette^Myr (dAbl^KN; Kette^Myr).