FIG. 4.

dAbl is a substrate of PTP61Fm. (A) HA-tagged dAbl was ectopically coexpressed with the Myc-tagged DA mutant form of PTP61Fm in S2 cells. Exogenous PTP61Fm was immunoprecipitated (IP) in the absence or presence of the substrate competitor sodium orthovanadate (Na₃VO₄; 10 mM). The immunocomplex or an aliquot of total lysates was analyzed by immunoblotting (IB). (B) S2 cells were treated with dsRNA for PTP61F or green fluorescent protein (GFP). dsRNA-treated S2 cells were then transfected with HA-tagged dAbl. Immunoprecipitated dAbl or an aliquot of total lysates was analyzed by immunoblotting. (C) The rough-eye phenotype caused by dAbl was enhanced in response to RNAi-mediated knockdown of PTP61F (i2-5, dAbl), whereas dAbl-induced eye roughness was rescued by cooverexpression of PTP61Fm (m2-2, dAbl). A magnified view of each genotype is shown to highlight the change in the organization of the ommatidial facets.