FIG. 2.

Generation of mice singly deficient for C2GnT2 or C2GnT3. (a) Gcnt3 genomic clone from 129/SvJ mouse strain was used to generate a targeting construct using the pflox vector as indicated. B, BglII; Ba, BamHI; E, EcoRI; S, SpeI; X, XbaI; Xh, XhoI. (b) Southern blotting of genomic DNA confirms the Gcnt3 allele structure present in ES cells using the genomic probe (top). Southern blotting with a loxP probe detects the location and number of loxP sites (bottom). (c) The targeting of the single coding exon of Gcnt4 using the pflox vector is depicted. A, AgeI; B, BamHI; E, EcoRV; S, SacI; Sa, SalI; St, StuI; X, XhoI. (d) Southern blots of genomic DNA with the genomic probe (top) or loxP probe (bottom) indicate the structure of the Gcnt4 alleles present. WT, wild type.