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. 2009 Apr 20;29(13):3803–3815. doi: 10.1128/MCB.00399-09

FIG. 2.

FIG. 2.

Gat1 conditions Gln3 binding to PGAP1. (A) Effects of gat1Δ and dal80Δ on Gln3-Myc13 binding to PGAP1. Glutamine- or proline-grown, untagged wild-type (wt), GLN3-MYC13 wild-type, gat1Δ, dal80Δ, and gat1Δ dal80Δ cells were analyzed by ChIP as described for Fig. 1A. (B) Effects of gat1Δ on Gln3-Myc13 subcellular localization. GLN3-MYC13 glutamine- or proline-grown wild-type and proline-grown gat1Δ cells were processed for indirect IF. Gln3-Myc13 and DNA were detected with anti-myc and DAPI (4′,6′-diamidino-2-phenylindole), respectively.