Table 1.
Selection of GCV-resistant cells transduced with ribozyme vector library
Transduction | Round
|
|
---|---|---|
1 | 2 | |
No ribozyme | 0 | 0 |
Control ribozyme | 8 | 72 ± 4 |
Ribozyme library | 38 ± 12 | 873 ± 228 |
Hygromycin B and GCV-resistant HeLa 5′tk cells were obtained after transduction with the retroviral hairpin ribozyme vector library or a control ribozyme vector. For additional rounds of selection, the ribozyme genes were PCR-amplified and were recloned into retroviral viral vectors for transduction of fresh HeLa 5′tk cells. Numbers are given as GCV-resistant colonies/150 cm2.