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. 2000 Jul 18;97(15):8566–8571. doi: 10.1073/pnas.97.15.8566

Table 1.

Selection of GCV-resistant cells transduced with ribozyme vector library

Transduction Round
1 2
No ribozyme 0 0
Control ribozyme 8 72  ± 4
Ribozyme library 38  ± 12 873  ± 228

Hygromycin B and GCV-resistant HeLa 5′tk cells were obtained after transduction with the retroviral hairpin ribozyme vector library or a control ribozyme vector. For additional rounds of selection, the ribozyme genes were PCR-amplified and were recloned into retroviral viral vectors for transduction of fresh HeLa 5′tk cells. Numbers are given as GCV-resistant colonies/150 cm2

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