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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Dev Cell. 2009 Jun;16(6):856–866. doi: 10.1016/j.devcel.2009.04.005

Figure 6. The interaction between caspase-2 and 14-3-3ζ is metabolically regulated.

Figure 6

(A) Upper panel: Glutathione sepharose-bound GST-C2 pro D137A was prephosphorylated with [γ-32P]ATP and incubated in extracts ± G6P or Bcl-xL. Samples of sepharose-bound proteins were analyzed by autoradiography. Lower panel: In parallel, extracts treated with the same conditions were analyzed for C3 activity using the caspase substrate Ac-DEVD-pNA. n = 3

(B) Radiolabeled in vitro translated full-length C2 WT or D137 was incubated in extracts ± G6P. Samples were retrieved over time and analyzed by SDS-PAGE and autoradiography.

(C) Glutathione sepharose-bound GST-C2 pro D137A was prephosphorylated with [γ-32P]ATP and incubated in buffer with His-PP1 that had been pre-incubated in extract. Samples of sepharose-bound proteins were analyzed by autoradiography. n = 2

(D) Percent survival of Xenopus oocytes microinjected with full-length C2 WT or D137A mRNA. n = 3