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. 1991 Apr;29(4):830–831. doi: 10.1128/jcm.29.4.830-831.1991

Acridine Orange Stain for Determining Intracellular Enteropathogens in HeLa Cells

Marianne D Miliotis 1
PMCID: PMC269882  PMID: 1716265

Abstract

Green-fluorescent intracellular enteropathogenic bacteria were observed after infected HeLa cell monolayers were stained with acridine orange and counterstained with crystal violet at least 3 h after infection.

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Selected References

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  1. Bhogale S. R., Sharma K. D., Kamat R. S. Role of heat labile antigens of Shigella flexneri in HeLa cell invasion. J Med Microbiol. 1983 Feb;16(1):37–43. doi: 10.1099/00222615-16-1-37. [DOI] [PubMed] [Google Scholar]
  2. Clerc P., Sansonetti P. J. Entry of Shigella flexneri into HeLa cells: evidence for directed phagocytosis involving actin polymerization and myosin accumulation. Infect Immun. 1987 Nov;55(11):2681–2688. doi: 10.1128/iai.55.11.2681-2688.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DuPont H. L., Formal S. B., Hornick R. B., Snyder M. J., Libonati J. P., Sheahan D. G., LaBrec E. H., Kalas J. P. Pathogenesis of Escherichia coli diarrhea. N Engl J Med. 1971 Jul 1;285(1):1–9. doi: 10.1056/NEJM197107012850101. [DOI] [PubMed] [Google Scholar]
  4. Evans D. J., Jr, Evans D. G. Three characteristics associated with enterotoxigenic Escherichia coli isolated from man. Infect Immun. 1973 Sep;8(3):322–328. doi: 10.1128/iai.8.3.322-328.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Giannella R. A., Formal S. B., Dammin G. J., Collins H. Pathogenesis of salmonellosis. Studies of fluid secretion, mucosal invasion, and morphologic reaction in the rabbit ileum. J Clin Invest. 1973 Feb;52(2):441–453. doi: 10.1172/JCI107201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Giannella R. A., Washington O., Gemski P., Formal S. B. Invasion of HeLa cells by Salmonella typhimurium: a model for study of invasiveness of Salmonella. J Infect Dis. 1973 Jul;128(1):69–75. doi: 10.1093/infdis/128.1.69. [DOI] [PubMed] [Google Scholar]
  7. Hale T. L., Bonventre P. F. Shigella infection of Henle intestinal epithelial cells: role of the bacterium. Infect Immun. 1979 Jun;24(3):879–886. doi: 10.1128/iai.24.3.879-886.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hale T. L., Sansonetti P. J., Schad P. A., Austin S., Formal S. B. Characterization of virulence plasmids and plasmid-associated outer membrane proteins in Shigella flexneri, Shigella sonnei, and Escherichia coli. Infect Immun. 1983 Apr;40(1):340–350. doi: 10.1128/iai.40.1.340-350.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kihlström E., Edebo L. Association of viable and inactivated Salmonella typhimurium 395 MS and MR 10 with HeLa cells. Infect Immun. 1976 Oct;14(4):851–857. doi: 10.1128/iai.14.4.851-857.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kihlström E. Infection of HeLa cells with Salmonella typhimurium 395 MS and MR10 bacteria. Infect Immun. 1977 Aug;17(2):290–295. doi: 10.1128/iai.17.2.290-295.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Labrec E. H., Schneider H., Magnani T. J., Formal S. B. EPITHELIAL CELL PENETRATION AS AN ESSENTIAL STEP IN THE PATHOGENESIS OF BACILLARY DYSENTERY. J Bacteriol. 1964 Nov;88(5):1503–1518. doi: 10.1128/jb.88.5.1503-1518.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lee W. H., McGrath P. P., Carter P. H., Eide E. L. The ability of some Yersinia enterocolitica strains to invade HeLa cells. Can J Microbiol. 1977 Dec;23(12):1714–1722. doi: 10.1139/m77-247. [DOI] [PubMed] [Google Scholar]
  13. Lindberg A. A. Bacterial virulence factors--with particular reference to Salmonella bacteria. Scand J Infect Dis Suppl. 1980;Suppl 24:86–92. [PubMed] [Google Scholar]
  14. Miliotis M. D., Koornhof H. J., Phillips J. I. Invasive potential of noncytotoxic enteropathogenic Escherichia coli in an in vitro Henle 407 cell model. Infect Immun. 1989 Jul;57(7):1928–1935. doi: 10.1128/iai.57.7.1928-1935.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Niesel D. W., Chambers C. E., Stockman S. L. Quantitation of HeLa cell monolayer invasion by Shigella and Salmonella species. J Clin Microbiol. 1985 Dec;22(6):897–902. doi: 10.1128/jcm.22.6.897-902.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ogawa H., Nakamura A., Sakazaki R. Pathogenic properties of "enteropathogenic" Escherichia coli from diarrheal children and adults. Jpn J Med Sci Biol. 1968 Oct;21(5):333–349. [PubMed] [Google Scholar]
  17. Okamura N., Nagai T., Nakaya R., Kondo S., Murakami M., Hisatsune K. HeLa cell invasiveness and O antigen of Shigella flexneri as separate and prerequisite attributes of virulence to evoke keratoconjunctivitis in guinea pigs. Infect Immun. 1983 Feb;39(2):505–513. doi: 10.1128/iai.39.2.505-513.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pruzanski W., Saito S., Nitzan D. W. The influence of lysostaphin on phagocytosis, intracellular bactericidal activity, and chemotaxis of human polymorphonuclear cells. J Lab Clin Med. 1983 Aug;102(2):298–305. [PubMed] [Google Scholar]
  19. Robins-Browne R. M., Jacobs M. R., Koornhof H. J. Escherichia coli gastro-enteritis in adults. S Afr Med J. 1978 Jan 21;53(3):93–94. [PubMed] [Google Scholar]
  20. Sansonetti P. J., Kopecko D. J., Formal S. B. Involvement of a plasmid in the invasive ability of Shigella flexneri. Infect Immun. 1982 Mar;35(3):852–860. doi: 10.1128/iai.35.3.852-860.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]

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