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Targeted transduction of CD20-positive human primary B cells. (a) Fresh unfractionated human PBMCs (1 × 106) were spin-transduced twice with indicated vectors (FUGW/αCD20+FM, 2.5 × 106 TU, or FUGW/VSVG, 25 × 106 TU). 48 hours later, cells were collected and analyzed by FACS (b) iMFI on fresh unfractionated human PBMCs transduced by the indicated viral vectors (FUGW/αCD20+FM, and FUGW/VSVG).
