Table 1.
Bacterial strains and plasmids used in this study
| Strains or plasmids | Relevant genotype and/or phenotype | Source or reference |
|---|---|---|
| V. cholerae | ||
| N16961 | Serogroup O1, El Tor biotype | Our lab store |
| N169-dtatABC | tatABC deletion mutant from N16961 | This study |
| N16961(pBAD24) | N16961 transformed with vector pBAD24 | This study |
| N169-dtatABC(pBAD24) | N169-dtatABC transformed with pBAD24 | This study |
| N169-dtatABC-cp | N169-dtatABC complemented with pBAD-TatABC | This study |
| N169-dtatABC-BCcp | N169-dtatABC complemented with pBAD-TatBC | This study |
| N169-dtatE | tatE deletion mutant from N16961 | This study |
| N169-dtatABCE | tatABC and tatE double deletion mutant from N16961 | This study |
| N169-dtatABCE-BCcp | N169-dtatABCE complemented with pBAD24 carrying tatBC | This study |
| N169-dtatB | tatB deletion mutant from N16961 | This study |
| N169-dtatC | tatC deletion mutant from N16961 | This study |
| E. coli | ||
| SM10 λpir | thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu Km | 21 |
| JARV16A (dtatAE) | tatA and tatE double deletion mutant from JARV16A | 34 |
| MCMTAA(dtatB) | tatB::Kan mutant from MCMTAA | 34 |
| B1LK0A (dtatC) | tatC deletion mutant from B1LK0A | 34 |
| DADEA (dtatABCDE) | tatABCD and tatE double deletion mutant from DADEA | 34 |
| Plasmids | ||
| pCVD442 | Suicide vector, ori R6K, Ampr, sacB | 21 |
| pDS132 | Suicide vector, ori R6K, from pCVD442, Cmr, sacB | 22 |
| pT1 | 714 bp EcoRI-KpnI fragment A of tatA cloned into pUC18 | This study |
| pT2 | 461 bp XbaI-PstI fragment B of tatC cloned into pT1 | This study |
| pT3 | 801 bp fragment of cat cloned into SmaI site of pT2 | This study |
| pCT4 | 1,976 bp fragment of 'A-cat-B' cloned into SphI site of pCVD442 | This study |
| pUC18C | intact tatABC and upstream fragment cloned between EcoRI and SacI site of pUC18 | This study |
| pBAD24 | pMB1-derived plasmid, Ampr, araBAD | 23 |
| pTatABC-301 | intact tatABC fragment of E. coli cloned into pBAD24 | This study |
| pBAD-TatABC | intact tatABC fragment of N16961 cloned into pBAD24 | This study |
| pBAD-TatBC | tatBC fragment of N16961 cloned into pBAD24 | This study |
| pBAD-TatE | tatE fragment of N16961 cloned into pBAD24 | This study |