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. 2009 May 13;9:92. doi: 10.1186/1471-2180-9-92

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Copyright ©2009 Zygmunt et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Regions and genes encoding LPS biosynthetic enzymes in B. melitensis 16 M Region wbk contains genes coding for: (i), enzymes necessary for N-formylperosamine synthesis (gmd, per, wbkC); (ii), two O-PS glycosyltransferase (wbkE, wbkA); (iii), the ABC transporter (wzm, wzt); (iv) the epimerase/dehydratase necessary for the synthesis of an N-acetylaminosugar (wbkD); and (v), the polyisoprenyl-phosphate N-acetylhexosamine-1-phosphate transferase enzyme that primes bactoprenol (wbkF). Genes manA_O-Ag, manB_O-Ag, manC_O-Agcould be involved in the synthesis of mannose, the perosamine precursor. Restriction sites: A, AluI; AvI, AvaI; Av, AvaII; B, BglI; Bg, BglII; C, ClaI; E, EcoRI; EV, EcoRV; H, HindIII; Ha, HaeII; Hf, HinfI; P, PstI; Pv, PvuII; S, Sau3A; Sa, SaII; St, StyI.