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. 2009 May 26;10:245. doi: 10.1186/1471-2164-10-245

Table 3.

E. amylovora TCTS mutants and their phenotypes

Gene/Operona Mutant designation Virulence assayb Amylovoran production (48 h) (OD600)c Significance levele Distance of swarming motility (48 h, cm)d Significance levele
WT +++ 0.08 ± 0.002 nop C 2.7 ± 0.15 cde
ams Z0118Δams --- 0.002 ± 0.0005 r ND ND ND
flhD Z2946ΔflhD +++ ND ND C 0.7 ± 0 w
fliA Z2986ΔfliA +++ ND ND C 0.7 ± 0 w
arcA Z1306ΔarcA +++ 0.053 ± 0.0001 qr C 1.23 ± 0.09 uv
arcB Z0117ΔarcB +++ 0.067 ± 0.0002 pq C 1.83 ± 0.28 qr
baeR Z3120ΔbaeR +++ 0.14 ± 0.045 klmn I 1.87 ± 0.25 qr
baeS Z3119ΔbaeS +++ 0.13 ± 0.03 klmn I 2.07 ± 0.06 nopq
baeSR Z3219–20ΔbaeSR +++ 0.1 ± 0.01 lmn I 2.07 ± 0.06 nopq
cpxA1 Z0686ΔcpxA1 +++ 0.09 ± 0.014 nop I 1.40 ± 0.22 tu
cpxA1R1 Z0686–87ΔcpxAR1 +++ 0.055 ± 0.003 pqr I 1.33 ± 0.05 uv
cpxR1 Z0687ΔcpxR1 +++ 0.08 ± 0.008 nop I 1.16 ± 0.13 v
cpxA2 Z3367ΔcpxA2 +++ 0.35 ± 0.023 hgi I 2.5 ± 0.4 efghi
cpxA2R2 Z3367–68ΔcpxAR2 +++ 0.29 ± 0.07 i I 2.07 ± 0.34 opqr
cpxR2 Z3368ΔcpxR2 +++ 0.35 ± 0.058 hgi I 2.5 ± 0.14 efgh
dcuR Z2361ΔdcuR +++ 0.198 ± 0.01 k I 2.2 ± 0.17 ijklm
dcuS Z2362ΔdcuS +++ 0.171 ± 0.02 kl I 2.07 ± 0.32 ijklm
dcuSR Z2361–62ΔdcuSR +++ 0.256 ± 0.02 j I 2.23 ± 0.38 mnop
eamI Z2195ΔeamI +++ 0.256 ± 0.046 j I 2.23 ± 0.12 ijklm
eamR Z2194ΔeamR +++ 0.544 ± 0.1 de I 2.03 ± 0.31 pqr
envZ Z0270ΔenvZ +++ 1.55 ± 0.06 c C 1.03 ± 0.11 v
envZ/ompR Z0270–71 ΔenvZ/ompR +++ 1.7 ± 0.05 a C 1.03 ± 0.11 v
ompR Z0271ΔompR +++ 1.63 ± 0.06 b C 1.03 ± 0.11 v
grrA Z2198ΔgrrA +++ 1.58 ± 0.15 bc C 3.15 ± 0.07 a
grrS Z3742ΔgrrS +++ 1.56 ± 0.09 c C 3.2 ± 0.05 a
hrpX Z3962ΔhrpX +++ 0.69 ± 0.11 d I 1.73 ± 0.15 rs
hrpY Z3963ΔhrpY +++ 0.471 ± 0.05 e I 2.0 ± 0.0 opqr
hrpXY Z3962–63 ΔhrpXY +++ 0.45 ± 0.09 ef I 2.33 ± 0.15 hijkl
hrpXYS Z3962–64 ΔhrpXYS --- 0.4 ± 0.09 gh I 2.27 ± 0.15 hijkl
kdpD Z0414ΔkdpD +++ 0.372 ± 0.02 hgi I 2.13 ± 0.12 nopq
kdpE Z1912ΔkdpE +++ 0.09 ± 0.035 no I 1.43 ± 0.19 tu
luxP Z3324ΔluxP +++ 0.419 ± 0.12 g I 1.87 ± 0.23 rs
luxQ Z3327ΔluxQ +++ 0.625 ± 0.11 de I 1.9 ± 0.17 qr
narP Z3390ΔnarP +++ 0.1 ± 0.001 lmn I 2.2 ± 0.0 klmno
narQ Z3389ΔnarQ +++ 0.08 ± 0.003 lmn I 2.4 ± 0.1 fghij
narQP Z3389–90 ΔnarQP +++ 0.08 ± 0.002 nop I 2.27 ± 0.25 ghijkl
phoB Z1675ΔphoB +++ 0.145 ± 0.003 klmn I 2.6 ± 0.2 defg
phoR Z1676ΔphoR +++ 0.366 ± 0.01 hgi I 2.75 ± 0.15 cd
phoRB Z1675–76 ΔphoRB +++ 0.312 ± 0.01 hi I 2.7 ± 0.1 cde
phoP Z2324ΔphoP +++ 0.15 ± 0.02 klm I 2.45 ± 0.17 cdef
phoQ Z2323ΔphoQ +++ 0.106 ± 0.01 lmno I 2.25 ± 0.23 efghi
phoQP Z2323–24 ΔphoQP +++ 0.18 ± 0.002 jk I 2.25 ± 0.15 ijklm
pmrA Z0089ΔpmrA +++ 0.05 ± 0.02 pqr I 2.3 ± 0.19 hijkl
pmrB Z0090ΔpmrB +++ 0.10 ± 0.01 lmno I 2.40 ± 0.17 defgh
pmrBA Z0089–90 ΔpmrBA +++ 0.07 ± 0.01 opq I 2.3 ± 0.20 fghij
rcsB Z3206ΔrcsB --- 0.002 ± 0.0005 r I 2.51 ± 0.17 efghi
rcsC Z3207ΔrcsC --- 0.4 ± 0.04 fg I 2.45 ± 0.2 fghij
rcsD (yojN) Z3205ΔrcsD --- 0.002 ± 0.0002 r I 2.48 ± 0.28 efghi
rcsDB Z3205–06 ΔrcsBD --- 0.003 ± 0.0001 r I 2.43 ± 0.11 fghijk
rstA Z2651ΔrstA +++ 0.359 ± 0.045 hgi I 1.6 ± 0 st
rstB Z2662ΔrstB +++ 0.1 ± 0.032 lmn I 2.03 ± 0.06 opqr
spk1 Z1250Δspk1 +++ 0.17 ± 0.04 kl I 2.58 ± 0.14 cdef
ybjN Z2074ΔybjN +++ 1.65 ± 0.12 a I 2.05 ± 0.26 lmno
yciR Z0955ΔyciR +++ 0.111 ± 0.0012 lmno I 1.9 ± 0.08 pqr
yddV Z1215ΔyddV +++ 0.043 ± 0.001 qr C 2.9 ± 0.14 bc
yegE Z3113ΔyegE +++ 0.22 ± 0.007 j I 2.9 ± 0.14 bc
yehT Z3996ΔyehU +++ 0.186 ± 0.026 k I 2.5 ± 0.2 efghi
yehU Z3995ΔyehT +++ 0.15 ± 0.04 klm I 2.33 ± 0.21 fghij
yehUT Z3995–96 ΔyehUT +++ 0.15 ± 0.004 klm I 2.4 ± 0.17 fghij
yfhA Z3498ΔyfhA +++ 0.07 ± 0.012 opq I 2.3 ± 0.1 hijkl
yfhK Z3495ΔyfhK +++ 0.06 ± 0.002 pq I 2.33 ± 0.25 fghij
yfhKA Z3495–98 ΔyfhKA +++ 0.062 ± 0.0005 pq I 2.17 ± 0.12 lmno
yoaD Z0355ΔyoaD +++ 0.22 ± 0.008 j I 2.4 ± 0.37 ghijkl

a ams operon encodes amylovoran biosynthesis. The entire operon (amsA-L; 15.8 kb) was deleted in the ams mutant [47]. flhD and fliA encode two regulators of flagella biosynthesis. These three mutants were used as negative controls for amylovoran biosynthesis and swarming motility, respectively.

b Virulence assay was performed using immature pear fruits as described. +++: pathogenic as WT; ---: non-pathogenic.

c Amylovoran production was measured 48 hr post inoculation. ND: not determined.

d Distance of swarming motility was measured 48 hr post inoculation. Original circle was about 0.6 cm in diameter. I: irregular movement; C: circular. ND: not determined.

e Values within the amylovoran production and distance of swarming motility columns followed by the same letter are not significantly different according to one way ANOVA and student-Newmans-Kleus test (P = 0.05).