Table 1.
Quantitative PCR measurements of selected voltage-gated K channels
| Channel | Type* | Rat gene symbol | Rat Gene ID | Abundance in control cells relative to Kv 1.1 |
Fold-change w/GRO/KC |
| Kv 1.1 | DR | Kcna1 | [GenBank: 24520] | 1.00 ± 0.17 | 1.18 ± 0.12 |
| Kv 1.2 | DR | Kcna2 | [GenBank: 25468] | 0.25 ± 0.04 | 1.24 ± 0.19 |
| Kv 1.3 | DR | Kcna3 | [GenBank: 29731] | 0.06 ± 0.04 | 0.81 ± 0.23 |
| Kv 1.4 | A-type | Kcna4 | [GenBank: 25469] | 0.16 ± 0.11 | 0.82 ± 0.22 |
| Kv 3.1 | DR | Kcnc1 | [GenBank: 25327] | 0.02 ± 0.00 | 1.14 ± 0.20 |
| Kv 3.4 | A-type | Kcnc4 | [GenBank: 684516] | 0.05 ± 0.00 | 1.11 ± 0.09 |
| Kv 4.3 | A-type | Kcnd3 | [GenBank: 65195] | 0.06 ± 0.01 | 0.89 ± 0.05 |
| MinK-2 | accessory | Kcne3 | [GenBank: 63883] | 0.02 ± 0.00 | 0.98 ± 0.15 |
N = 4 cultures from 4 animals, each of which provided a pair of RNA samples (control cells and GRO/KC treated cells). All expression values were normalized to that of the housekeeping gene HPRT from the same sample; fold changes (GRO/KC/control) were calculated for each individual pair of matched samples. For comparison of relative abundance of channel types in control cells, expression data were normalized to the most abundant channel observed, Kv1.1. None of the fold-change values after overnight GRO/KC incubation was significantly different from 1.
*, channel type as given by reference [26], "DR" = delayed rectifier; however, K channel properties may also depend on accessory proteins and heteromer composition.