Figure 5.

shRNA target sites mapped to the 13 fluorescent reporters. The target sites of the 96 shRNAs were mapped to the 13 fluorescent reporters. The 4 short accessory genes and LTR reporters are shown down the left, and the 3 long core gene reporters are shown down the right. Mapped onto the long core gene reporters were the corresponding 6 short gene fragment reporters made to retest the core gene shRNAs. Lengths are to scale to show the relative distances between the target sites, fusion junctions and total reporter lengths. n.b. some shRNAs were assayed with a different reporter to that of the gene targeted due to overlapping target sequences, e.g. the Vif matched shRNAs #51 – 57 were assayed with Pol reporters.