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. 2000 Jul 4;97(15):8572–8577. doi: 10.1073/pnas.140109997

Figure 3.

Figure 3

The restriction map of the CTXET prophage in AS207 differs from the map of pCTXET found in transductants derived from AS207. (A) Restriction map of AS207 chromosomal DNA. N, NotI; P, PstI. (B) A Southern blot of donor chromosomal DNA and plasmid DNA from potential transductants probed with rstRET (underlined in A). This probe detected a 2.9-kb band in a NotI/PstI digest of AS207 chromosomal DNA (lane 1) but a 7-kb band in a NotI/PstI digest (lane 3) of plasmid DNA from a pool of potential transductants. Digestion of the plasmid DNA with PstI alone (lane 4) revealed that the NotI site (shaded in A), rather than the PstI site, was absent from the plasmid. No hybridization was observed to NotI/PstI-digested plasmid DNA isolated from the transduction recipient O395 grown without donor supernatant (lane 2).