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A solitary prophage with a fragment of RS1 inserted downstream can efficiently produce CTX virions. BD555 is identical to BD305 except that it contains a CTXETNF-Kn prophage (derived from pCTXETNF-Kn), not a CTXET-Kn prophage. KnR transductants derived from BD555 always contain pCTXET-Kn. The inserted fragment in BD328 is too small to rescue the 5′ prophage.
