Fig. 6.

Steady-state glucuronidation of CBN (A), THC-OH (B), and THC-COOH (C) with selected recombinant UGT isoforms. Glucuronidation activities of recombinant proteins were measured by incubating membrane fractions containing recombinant UGT1A10 (5 μg) with increasing concentrations (shown in the figure) of substrate at a constant concentration of UDP-GlcUA (4 mM). Curve fits and kinetic constants were determined using GraphPad Prism 4 software, and the resulting parameters are included in Table 2.