TABLE 1.
MS/MS experimental conditions for product ion, multiple reaction monitoring, and neutral loss studies
| Analyte | Q1 | Q3 | CE | EP | DP | CXP | |
|---|---|---|---|---|---|---|---|
| m/z | V | ||||||
| Product ion | |||||||
| 1 | CBN-Gluc | 485.1 | 50-525 | -20 to -35 | -10 | -50 to -70 | -9 to -15 (2 s) |
| 2 | THC-O-Gluc | 505.3 | 50-510 | -30 to -40 | -10 | -50 to -70 | -9 |
| 3 | THC-COO-Gluc | 519.3 | 50-525 | -30 to -40 | -10 | -50 to -70 | -9 |
| MRM | |||||||
| 1 | CBN | 309.3 | 171.1 | -40 | -10 | -89 | -3 |
| 309.3 | 279.2 | -42 | -10 | -91 | -7 | ||
| 2 | Δ9-THC and CBD | 313.3 | 191.2 | -38 | -10 | -87 | -3 |
| 313.3 | 245.3 | -36 | -10 | -82 | -5 | ||
| 3 | THC-OH | 329.3 | 268.3 | -38 | -10 | -69 | -6 |
| 329.3 | 311.2 | -25 | -10 | -65 | -8 | ||
| 4 | THC-COOH | 343.2 | 245.2 | -40 | -10 | -82 | -5 |
| 343.2 | 299.3 | -29 | -10 | -75 | -7 | ||
| 5 | CBN-Gluc | 485.1 | 113.2 | -29 | -10 | -61 | -8 |
| 485.1 | 309.2 | -27 | -10 | -76 | -8 | ||
| 6 | Δ9-THC-Gluc and CBD-Gluc | 489.3 | 313.1 | -35 | -10 | -61 | -8 |
| 489.3 | 374.9 | -30 | -10 | -76 | -9 | ||
| 7 | THC-O-Gluc | 505.3 | 311.2 | -44 | -10 | -56 | -8 |
| 505.3 | 329.2 | -31 | -10 | -59 | -8 | ||
| 8 | THC-COO-Gluc | 519.3 | 193.2 | -25 | -10 | -47 | -8 |
| 519.3 | 343.3 | -33 | -10 | -36 | -9 | ||
| Neutral | Loss of 176 | ||||||
| Loss | |||||||
| 1 | Loss of glucuronic acid | (400-800 amu) | -20 to -35 | -10 | -50 to -80 | -9 to -15 (2 s) | |
CE, collision energy; EP, entrance potential; DP, declustering potential; CXP, collision cell exit potential; Gluc, glucuronide; MRM, multiple reaction monitoring.