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. Author manuscript; available in PMC: 2010 Mar 4.
Published in final edited form as: J Control Release. 2008 Nov 17;134(2):81–90. doi: 10.1016/j.jconrel.2008.10.021

Figure 5.

Figure 5

Histological analysis on silk scaffolds with rhBMP-2/rhIGF-I reverse gradient. A–F, the first scaffold segment (lowest rhBMP-2 and highest rhIGF-I). G–L, the seventh scaffold segment (highest rhBMP-2 and lowest rhIGF-I). A,D,G,J, Hematoxylin and eosin (H&E) staining. B,E,H,K, Alcian blue staining for proteoglycan formation. C,F,I,L, Von Kossa staining for calcium deposition. Scale bars = 200 μm (A–C, G–I) or 50 μm (D–F, J–L)