Figure 4. LA-induced mitochondrial Ca²⁺ efflux was responsible for peroxynitrite generation.

Mitochondria were prepared from HM cells and labeled with X-rhod-1 (2 µM) and DCF (1 µM) with (A) or without Ca²⁺ (B). LA-induced mitochondrial Ca²⁺ efflux (black traces) and peroxynitrite generation (red traces) were indexed by the gradual decrease in X-rhod-1 and increase in DCF fluorescence intensity, respectively.