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. Author manuscript; available in PMC: 2010 May 15.
Published in final edited form as: Cell. 2009 May 15;137(4):672–684. doi: 10.1016/j.cell.2009.03.035

Figure 2. Correlation of Delta Values with Centromere Stretch: the Ndc80 Complex Is Not Compliant and Maintains a Constant Shape.

Figure 2

(A) Schematic of sister kinetochore pairs in different mechanical states (top); Predictions of Delta measurements for non-compliant (middle) and compliant (bottom) protein linkages. (B) Delta values of separations between Hec1 9G3 and antibody to Spc24 C-terminus across the entire range of K-K distances. (C) Delta values for 9G3 anti-Hec1 labeling with a mixture of red/green fluorescent secondary antibodies are individually plotted versus K-K distance. For (B) and (C), measurements are insensitive to K-K separation. (D) Delta values of separations between Hec1 9G3 and GFP-CENP-A across the entire range of K-K distances show oscillation compliance. The whole dataset of Delta values uncorrected for tilt are shown; average Deltas are within 1–2 nm of corrected values (Supp. Table 1).