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. 2000 Jul 11;97(15):8584–8589. doi: 10.1073/pnas.140222497

Figure 2.

Figure 2

BDNF expression in the HVC of adult male canaries. (A) Dark-field images of in situ hybridization, showing BDNF mRNA expression in HVC of singing and nonsinging birds. (Scale bar, 200 μm.) (B) Quantitative analysis of BDNF mRNA expression in the HVC and hippocampus of nonsinging (n = 4) and singing birds (n = 6), P < 0.0001 for HVC and P = 0.97 for hippocampus, unpaired t test. Values are mean ± SEM. (C) Western blot analysis showing the amount of BDNF protein in the HVC of singing birds (n = 7) is 30% higher than that in the nonsinging birds (n = 6); P < 0.0001, unpaired t test.